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Proteinase K powder (20mg/vial, 30U/mg)

This product is supplied in a freeze-dried format, allowing easy reconstitution by adding Nuclease-free water for immediate use. Store at 4°C for short-term use or aliquot and

store at -20°C. Immediate use is recommended.

This product is classified as a serine protease with endolytic activity, specifically proteinase K. It is employed to minimize protein contamination and enhance DNA/RNA extraction efficiency in nucleic acid extraction experiments.

Cat.# : IN4002

Clear-S® Total RNA

extraction kit

Clear-S® Total RNA Extraction Kit 는 세포 및 동물 조직, 식물 조직, 곤충 등 다양 한 시료에서 깨끗하게 total RNA를 추출할 수 있는 제품입니다.

Genomic DNA 오염 없이 쉽고 빠르게 total RNA를 추출하며 실험 간 오차를 줄여 재현성 높은 결과를 얻도록 설계된 spin column type의 제품입니다.

◎ 특징

다양한 시료에서 신속하고 효과적으로 고품질 total RNA 추출

Genomic DNA contamination 최소화

Spin-column 사용으로 높은 재현성 획득

Phenol, chloroform 없이 핵산추출가능

◎ 응용 및 적용

cDNA synthesis, RT-PCR, Quantitative Real-time RT-PCR, Digital RT-PCR, Northern Blot

Product information

• Serine Protease Enzyme: Our product belongs to the serine protease class, which has a proven track record of facilitating enzymatic reactions that result in the breakdown of proteins. This attribute ensures optimal disruption of protein structures in your samples, leading to improved nucleic acid release.

• Enhanced DNA/RNA Extraction: The inclusion of our Serine Protease in your nucleic acid extraction workflow enhances the overall efficiency of DNA and RNA extraction. The enzyme's targeted action on proteins minimizes interference, resulting in purer and more concentrated nucleic acid yields.

• Sample Versatility: Our Serine Protease is compatible with a wide range of sample types, making it an adaptable solution for various research applications. Whether you're working with challenging tissue samples or delicate cell cultures, this enzyme assists in obtaining reliable nucleic acid results.

• Quality and Purity: We understand the importance of high-purity nucleic acids for downstream applications. Our Serine Protease aids in achieving superior purity by effectively eliminating protein contaminants that can interfere with your analysis.

• Easy Integration: Incorporating our Serine Protease into your existing nucleic acid extraction protocols is straightforward. Its compatibility with common extraction reagents and methods ensures a seamless integration process.

Revolutionize your nucleic acid extraction processes with our advanced Serine Protease. Elevate the efficiency and accuracy of your DNA and RNA isolation, and empower your research with the high-quality nucleic acids needed for success.

Usage Instructions:

1.Prepare your sample according to your standard protocol.
2.Add the recommended amount of Serine Protease to your sample preparation buffer.
3.Incubate the mixture at the specified temperature and duration to allow efficient protein degradation.
4.Proceed with your nucleic acid extraction process as usual, taking advantage of the improved sample solubilization achieved by our Serine Protease.

Transform your nucleic acid extraction experience today with our Serine Protease. Elevate your results, enhance your workflow, and gain the confidence you need in your research endeavors.

Buffer (pH = 8.0, 50 °C, 1.25 µg/mL protease K, 15 min incubation)

Proteinase K activity (%)

30 mM Tris·Cl

100

30 mM Tris·Cl; 30 mM EDTA; 5% Tween 20; 0.5% Triton X-100; 800 mM GuHCl

313

36 mM Tris·Cl; 36 mM EDTA; 5% Tween 20; 0.36% Triton X-100; 735 mM GuHCl

301

10 mM Tris·Cl; 25 mM EDTA; 100 mM NaCl; 0.5% SDS

128

10 mM Tris·Cl; 100 mM EDTA; 20 mM NaCl; 1% Sarkosyl

10 mM Tris·Cl; 50 mM KCl; 1.5 mM MgCl2; 0.45% Tween 20; 0.5% Triton X-100

106

10 mM Tris·Cl; 100 mM EDTA; 0.5% SDS

120

30 mM Tris·Cl; 10 mM EDTA; 1% SDS

203

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